Cell Services

MingCelerFeeder Cells

Product Description

Ready-to-Use Feeder Cells for Robust Stem Cell Culture

Eliminate the inconsistency and labor-intensive process of preparing your own feeder layers. Our pre-tested, mitomycin-C-treated or gamma-irradiated feeder cells provide a reliable foundation for your most sensitive cultures. They are designed to secrete necessary nutrients and create an optimal microenvironment, maximizing plating efficiency and supporting long-term stem cell growth while minimizing spontaneous differentiation.

Why Choose Our Feeders?

Save Time & Resources: Avoid the hassle of primary cell isolation and inactivation.
Reduce Variability: Achieve more reproducible results with our highly consistent batches.
Expert Support: Backed by our team's extensive experience in cell culture applications.

Product Features

01

High Nourishing Capacity

The cultured ESC/iPSC colonies exhibit a characteristic dome-shaped morphology with well-defined edges, transparent and bright cytoplasm, and tight cell-cell connections, showing no signs of spontaneous differentiation. In the field of embryonic stem cell (ES cell) research, the tetraploid complementation assay is considered the gold standard for validating ES cell quality. When ES cells cultured on feeder cells prepared by Mingxin Biotechnology are subjected to the tetraploid complementation assay, the live birth rate of mice reaches 30%-60%, significantly surpassing the reported rates of only 1%-5% in existing literature.
02

Pre-inactivated Treatment

The feeder cells are pre-inactivated with Mitomycin C (Mito-C), allowing for immediate use upon thawing. This pre-treatment saves researchers at least two weeks of preparation time. The treated cells are fully inactivated and exhibit almost no proliferation, thereby providing a stable growth environment for embryonic stem cell culture.
03

Rich Selection of Resistance Markers

This feeder cell line contains a variety of single and combined resistance markers, including Neo, Puro, BSD, and Hygro, catering to diverse experimental requirements. This allows researchers to select the most suitable feeder cells based on their specific experimental needs, thereby enhancing experimental flexibility and success rates.
04

High Post-Thaw Viability

The post-thaw attachment rate of the cells exceeds 80%, with the cells exhibiting a healthy morphology characterized by a three-dimensional, elongated spindle shape. This high post-thaw viability and optimal cellular morphology ensure that the feeder cells can rapidly fulfill their supportive function for embryonic stem cell growth, providing a reliable foundation for embryonic stem cell culture.

Feeder Cell Preparation Process

The preparation process begins with the isolation of embryonic tissue from pregnant mice at 13.5 days post-coitum. Primary Mouse Embryonic Fibroblasts (MEF cells) are obtained through a series of separation and purification steps, designated as passage 0 (P0). These P0 MEF cells are then expanded through subsequent passages. Following expansion, the cells are treated with Mitomycin C to inhibit their proliferative capacity. At this stage, the treated MEF cells are classified as Feeder cells.

The prepared Feeder cells are then cryopreserved and stored in liquid nitrogen tanks for long-term preservation. Throughout the preparation process, rigorous quality control measures are implemented, including assessments of cell morphology, viability testing, and mycoplasma detection.

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